Material Information |
| Title: |
Characterization of Spermatozoal Small Non-coding RNAs using Functional Genomics and Immunocytochemistry |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
Crate, Elizabeth |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2013 |
| Publication Date: |
2013 |
Subjects |
| Subjects / Keywords: |
Immunocytochemistry
Small Non-Coding RNAs
Functional Genomics |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
Fertilization is the cornerstone of early mammalian development which leads to formation of zygote and then embryo. In addition to providing half of the zygotic genome, the sperm also contributes macromolecules such as small non-coding RNAs (sncRNAs) into the egg. However, identities of these paternal sncRNAs, as well as molecular and cellular mechanisms by which these molecules regulate embryonic development, are not well understood. This gap in the knowledge base is an important problem because it is preventing advancement in developmental biology and reproductive biotechnology. The central hypothesis is that sperm contain diverse sets of sncRNAs, including microRNAs (miRNAs) that play vital roles in embryonic genome activation at the onset of mammalian development. The overall approach was a comprehensive literature review followed by experiments using miRNA microarray to validate the expression of a series of miRNAs in sperm from bulls of high and low fertility. For my portion of this larger project, immunocytochemistry was employed for the examination of PIWI proteins and AGO2 protein in mature sperm and RNA isolation and bioinformatics was used to survey differences in miRNA expression between high and low fertility bull samples. Overall the results showed that: 1) sperm contain ample amounts of sncRNAs, including miRNAs, that may be essential for embryo development 2) proteins that bind to the sncRNAs, such as PIWIL1, PIWIL2 and AGO2, are well-conserved across mammals and appear to be present in the mature bull sperm. The findings may ultimately be important for better understanding of dynamic molecular and events in sperm and embryos that are critical for mammalian development, and for developing reproductive technologies such as biomarkers for predicting male fertility. |
| Statement of Responsibility: |
by Elizabeth Crate |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2013 |
| General Note: |
Online version not currently available. |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Clore, Amy |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2013 C89 |
| System ID: |
NCFE004739:00001 |
|
Material Information |
| Title: |
Characterization of Spermatozoal Small Non-coding RNAs using Functional Genomics and Immunocytochemistry |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
Crate, Elizabeth |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2013 |
| Publication Date: |
2013 |
Subjects |
| Subjects / Keywords: |
Immunocytochemistry
Small Non-Coding RNAs
Functional Genomics |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
Fertilization is the cornerstone of early mammalian development which leads to formation of zygote and then embryo. In addition to providing half of the zygotic genome, the sperm also contributes macromolecules such as small non-coding RNAs (sncRNAs) into the egg. However, identities of these paternal sncRNAs, as well as molecular and cellular mechanisms by which these molecules regulate embryonic development, are not well understood. This gap in the knowledge base is an important problem because it is preventing advancement in developmental biology and reproductive biotechnology. The central hypothesis is that sperm contain diverse sets of sncRNAs, including microRNAs (miRNAs) that play vital roles in embryonic genome activation at the onset of mammalian development. The overall approach was a comprehensive literature review followed by experiments using miRNA microarray to validate the expression of a series of miRNAs in sperm from bulls of high and low fertility. For my portion of this larger project, immunocytochemistry was employed for the examination of PIWI proteins and AGO2 protein in mature sperm and RNA isolation and bioinformatics was used to survey differences in miRNA expression between high and low fertility bull samples. Overall the results showed that: 1) sperm contain ample amounts of sncRNAs, including miRNAs, that may be essential for embryo development 2) proteins that bind to the sncRNAs, such as PIWIL1, PIWIL2 and AGO2, are well-conserved across mammals and appear to be present in the mature bull sperm. The findings may ultimately be important for better understanding of dynamic molecular and events in sperm and embryos that are critical for mammalian development, and for developing reproductive technologies such as biomarkers for predicting male fertility. |
| Statement of Responsibility: |
by Elizabeth Crate |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2013 |
| General Note: |
Online version not currently available. |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Clore, Amy |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2013 C89 |
| System ID: |
NCFE004739:00001 |
|