Material Information
- Title:
- Exogenous Expression of DNA Pilot Protein H with Mutated Colied-Coil Domains Inhibits Bacteriophage ?X174
- Physical Description:
- Book
- Language:
- English
- Creator:
- Young, Lindsey
- Publisher:
- New College of Florida
- Place of Publication:
- Sarasota, Fla.
- Creation Date:
- 2011
- Publication Date:
- 2011
Thesis/Dissertation Information
- Degree:
- Bachelor's ( B.A.)
- Degree Grantor:
- New College of Florida
- Degree Divisions:
- Natural Sciences
- Area of Concentration:
- Chemistry, Biology
Subjects
- Subjects / Keywords:
- Virus
Colied-Coils
Biochemistry
- Genre:
- Electronic Thesis or Dissertation
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
Notes
- Abstract:
- The DNA pilot protein H of bacteriophage ?X174 is known to pilot the viral genome into the host organism, Escherichia coli. Recent evidence suggests the DNA pilot protein is also involved in protein biosynthesis. Though the structure of the DNA pilot protein is unknown, it is predicted by bioinformatics to have an N-terminal domain, four coiled-coil domains, and two DNA binding sites. The sequence of the DNA pilot protein was cloned with mutations that disrupted coils 1 and 4 of the protein. Another set of mutants contained an N-terminal deletion starting at residue 142 in addition to the mutations that disrupted the coiled-coil domains. The mutagenic genes were exogenously expressed in vivo under lac induction and found to inhibit viral formation of bacteriophage ?X174 and inhibit viral protein synthesis. Three point mutations in the sequence of viral H reduced the inhibitory effects of the exogenous mutagenic DNA pilot proteins. The point mutations were identified as V286L and S284F/Y. Thus, it is possible that the DNA pilot protein plays an additional role in transcription and/or translation, however, further evidence is necessary to state this definitively. The laboratory work for this undergraduate thesis was completed at the University of Arizona under the guidance of Dr. Bentley Fane.
- Thesis:
- Thesis (B.A.) -- New College of Florida, 2011
- Electronic Access:
- RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
- Bibliography:
- Includes bibliographical references.
- Source of Description:
- This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
- Local:
- Faculty Sponsor: Walstrom, Katherine
- Statement of Responsibility:
- by Lindsey Young
Record Information
- Source Institution:
- New College of Florida
- Holding Location:
- New College of Florida
- Rights Management:
- Applicable rights reserved.
- Classification:
- local - S.T. 2011 Y7
- System ID:
- NCFE004520:00001
|
