Material Information |
| Title: |
The Identification of Cocaine-Induced Changes in the Human Monocyte Proteome Using Isotope-Coded Affinity Tags |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
Phillips, John W. |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2008 |
| Publication Date: |
2008 |
Subjects |
| Subjects / Keywords: |
ICAT
Proteomics
Cocaine
Mass Spectronetry
Monocyte |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
Cocaine is a widely abused drug with a broad spectrum of physiological effects. In addition to its addictive properties, cocaine has been shown to result in suppression of the immune system. In recent years, the cellular mechanisms underlying drug response and disease states have been investigated using liquid chromatography (LC) and tandem mass spectrometry (MS/MS) to map changes in protein expression in affected cells. A new technique, utilizing isotope-coded affinity tags (ICAT), allows quantitative as well as qualitative changes in protein expression to be determined by LC/MS/MS. This study sought to characterize the effects of cocaine exposure on protein expression in cultured human monocytes using these methods to investigate the mechanisms underlying cocaine-induced immunosuppression. Two proteins thought to be involved in inflammatory immune responses, pannexin 3 and macrophage migration inhibitory factor (MIF), showed decreases in abundance as a result of cocaine exposure. In addition, two proteins involved in protein synthesis, eukaryotic translation elongation factor 2(EF-2) and a homologue of 60S ribosomal protein L12, showed decreases in abundance. These results are consistent with previous findings that cocaine causes inhibition of the inflammatory immune response. A novel mechanism for cocaine-induced immunosuppression, mediated via the antagonism of muscarinic acetylcholine receptors, is proposed. |
| Statement of Responsibility: |
by John W. Phillips |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2008 |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Walstrom, Katherine |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2008 P55 |
| System ID: |
NCFE003990:00001 |
|
Material Information |
| Title: |
The Identification of Cocaine-Induced Changes in the Human Monocyte Proteome Using Isotope-Coded Affinity Tags |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
Phillips, John W. |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2008 |
| Publication Date: |
2008 |
Subjects |
| Subjects / Keywords: |
ICAT
Proteomics
Cocaine
Mass Spectronetry
Monocyte |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
Cocaine is a widely abused drug with a broad spectrum of physiological effects. In addition to its addictive properties, cocaine has been shown to result in suppression of the immune system. In recent years, the cellular mechanisms underlying drug response and disease states have been investigated using liquid chromatography (LC) and tandem mass spectrometry (MS/MS) to map changes in protein expression in affected cells. A new technique, utilizing isotope-coded affinity tags (ICAT), allows quantitative as well as qualitative changes in protein expression to be determined by LC/MS/MS. This study sought to characterize the effects of cocaine exposure on protein expression in cultured human monocytes using these methods to investigate the mechanisms underlying cocaine-induced immunosuppression. Two proteins thought to be involved in inflammatory immune responses, pannexin 3 and macrophage migration inhibitory factor (MIF), showed decreases in abundance as a result of cocaine exposure. In addition, two proteins involved in protein synthesis, eukaryotic translation elongation factor 2(EF-2) and a homologue of 60S ribosomal protein L12, showed decreases in abundance. These results are consistent with previous findings that cocaine causes inhibition of the inflammatory immune response. A novel mechanism for cocaine-induced immunosuppression, mediated via the antagonism of muscarinic acetylcholine receptors, is proposed. |
| Statement of Responsibility: |
by John W. Phillips |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2008 |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Walstrom, Katherine |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2008 P55 |
| System ID: |
NCFE003990:00001 |
|