Material Information |
| Title: |
X-Linked Gene Expression in Wild-Type, rha-1(tm329), and rde-2(ne221) C. elegans Using Realt-Time Reverse Transcription Polymerase Chain Reaction |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
White, Justin |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2007 |
| Publication Date: |
2007 |
Subjects |
| Subjects / Keywords: |
C. elegans
RHA-1
Real-Time RT PCR |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
Helicases are enzymes that separate dsRNA or dsDNA and have been implicated in several different cellular processes. In particular, RNA helicase A (RHA) is a multifunctional helicase involved in transcription regulation and histone modification. A deletion in rha-1 in C. elegans produces a temperature sensitive sterile mutant (Walstrom et al., 2005). The mutant worms display a different series of histone modification and of particular interest is the loss of the histone 3 dimethylation on lysine 9. Rha- 1 also plays a role in the conserved RNA interference (RNAi) pathway, which is a post-transcriptional silencing pathway. Another gene required for RNAi is rde-2. We used a null mutant of rde-2 and the rha-1 mutant to determine the respective effects of the mutations on genetic expression of X-linked genes in the germline. Using Real-time RT-PCR, we discovered that there was little change the expression of many of the X-linked genes examined. There was more expression in the rha-1 mutants than in the rde- 2 mutants, which can be explained by the presence of the H3K9 methylation (silencing modification) in the rde-2 mutants but not in the rha-1 mutants. There seemed to be a defect in silencing with respect to gene T08D2.4 in both mutant strains. After examining the gene expression results, a double mutant was constructed. |
| Statement of Responsibility: |
by Justin White |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2007 |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Walstrom, Katherine |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2007 W5 |
| System ID: |
NCFE003869:00001 |
|
Material Information |
| Title: |
X-Linked Gene Expression in Wild-Type, rha-1(tm329), and rde-2(ne221) C. elegans Using Realt-Time Reverse Transcription Polymerase Chain Reaction |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
White, Justin |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2007 |
| Publication Date: |
2007 |
Subjects |
| Subjects / Keywords: |
C. elegans
RHA-1
Real-Time RT PCR |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
Helicases are enzymes that separate dsRNA or dsDNA and have been implicated in several different cellular processes. In particular, RNA helicase A (RHA) is a multifunctional helicase involved in transcription regulation and histone modification. A deletion in rha-1 in C. elegans produces a temperature sensitive sterile mutant (Walstrom et al., 2005). The mutant worms display a different series of histone modification and of particular interest is the loss of the histone 3 dimethylation on lysine 9. Rha- 1 also plays a role in the conserved RNA interference (RNAi) pathway, which is a post-transcriptional silencing pathway. Another gene required for RNAi is rde-2. We used a null mutant of rde-2 and the rha-1 mutant to determine the respective effects of the mutations on genetic expression of X-linked genes in the germline. Using Real-time RT-PCR, we discovered that there was little change the expression of many of the X-linked genes examined. There was more expression in the rha-1 mutants than in the rde- 2 mutants, which can be explained by the presence of the H3K9 methylation (silencing modification) in the rde-2 mutants but not in the rha-1 mutants. There seemed to be a defect in silencing with respect to gene T08D2.4 in both mutant strains. After examining the gene expression results, a double mutant was constructed. |
| Statement of Responsibility: |
by Justin White |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2007 |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Walstrom, Katherine |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2007 W5 |
| System ID: |
NCFE003869:00001 |
|