Material Information |
| Title: |
Investigation of Caenorhabditis elegans RNA Helicase A Protein Binding with a Yeast 2-Hybrid Assay |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
Summers, Carly |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2005 |
| Publication Date: |
2005 |
Subjects |
| Subjects / Keywords: |
Yeast 2-Hybrid
Helicase
Protein |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
RNA helicase A (RHA) is a highly conserved helicase protein with established roles in nucleic acid binding and transcription regulation. In order to better understand the function of RHA in the model organism C. elegans, a yeast 2-hybrid assay was performed to identify proteins that interact with RHA-1, the C. elegans form of RHA. A p-ACT RB2 randomprimed C. elegans cDNA library was used to perform this screen within the yeast strain PJ69-4A. One protein in the screen was found to specifically interact with RHA-1. This protein is calnexin (CNX-1), a molecular chaperone currently thought to be localized to the endoplasmic reticulum. The probability and implications of a RHA 1/CNX-1 interaction are discussed in the context of the results of this experiment as well as literature related to the proteins of interest. |
| Statement of Responsibility: |
by Carly Summers |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2005 |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Walstrom, Katherine |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2005 S9 |
| System ID: |
NCFE003576:00001 |
|
Material Information |
| Title: |
Investigation of Caenorhabditis elegans RNA Helicase A Protein Binding with a Yeast 2-Hybrid Assay |
| Physical Description: |
Book |
| Language: |
English |
| Creator: |
Summers, Carly |
| Publisher: |
New College of Florida |
| Place of Publication: |
Sarasota, Fla. |
| Creation Date: |
2005 |
| Publication Date: |
2005 |
Subjects |
| Subjects / Keywords: |
Yeast 2-Hybrid
Helicase
Protein |
| Genre: |
bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, terriorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation |
Notes |
| Abstract: |
RNA helicase A (RHA) is a highly conserved helicase protein with established roles in nucleic acid binding and transcription regulation. In order to better understand the function of RHA in the model organism C. elegans, a yeast 2-hybrid assay was performed to identify proteins that interact with RHA-1, the C. elegans form of RHA. A p-ACT RB2 randomprimed C. elegans cDNA library was used to perform this screen within the yeast strain PJ69-4A. One protein in the screen was found to specifically interact with RHA-1. This protein is calnexin (CNX-1), a molecular chaperone currently thought to be localized to the endoplasmic reticulum. The probability and implications of a RHA 1/CNX-1 interaction are discussed in the context of the results of this experiment as well as literature related to the proteins of interest. |
| Statement of Responsibility: |
by Carly Summers |
| Thesis: |
Thesis (B.A.) -- New College of Florida, 2005 |
| Electronic Access: |
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE |
| Bibliography: |
Includes bibliographical references. |
| Source of Description: |
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. |
| Local: |
Faculty Sponsor: Walstrom, Katherine |
Record Information |
| Source Institution: |
New College of Florida |
| Holding Location: |
New College of Florida |
| Rights Management: |
Applicable rights reserved. |
| Classification: |
local - S.T. 2005 S9 |
| System ID: |
NCFE003576:00001 |
|