Material Information
- Title:
- THE COMPETITION BETWEEN microRNA 495 AND HuD AND ITS EFFECTS ON THE REGULATION OF mRNA TARGETS INVOLVED IN COCAINE ADDICTION
- Physical Description:
- Book
- Language:
- English
- Creator:
- Quezada, Alexandra
- Publisher:
- New College of Florida
- Place of Publication:
- Sarasota, Fla.
- Publication Date:
- 2014
Thesis/Dissertation Information
- Degree:
- Bachelor's ( B.A.)
- Degree Grantor:
- New College of Florida
- Degree Divisions:
- Natural Sciences
- Area of Concentration:
- Neurobiology
Subjects
- Genre:
- Electronic Thesis or Dissertation
bibliography ( marcgt )
theses ( marcgt )
Notes
- Abstract:
- RNA-binding protein, HuD, is necessary for proper neuronal development in mammals and is also involved in neuronal growth during synaptic plasticity. HuD also acts as a trans-acting factor by stabilizing mRNA transcripts in the cytoplasm and promoting translation. MicroRNA-495 (miR-495) acts as a destabilizing trans-acting factor by promoting deadenylation or repression mechanisms. HuD and miR-495 share homologous sequences in the RNA binding domains of mRNA targets, making them competitors. Many of the mRNA targets of HuD and miR-495 are addiction-related genes involved in cocaine addiction. To see the effect of HuD and miR-495 competition on addiction related-genes, we used a mouse model overexpressing HuD (HuD Tg). The relative levels of brain-derived neurotrophic factor (BDNF), HuD, and miR-495 in the nucleus accumbens were quantified using real time polymerase chain reaction, after a sub- chronic treatment of cocaine. The relative RNA levels of miR-495 were increased in the HuD Tg and the control mice; all other data were insignificant. This pilot study can help mold further studies on the competition between HuD and miR-495.
- Thesis:
- Thesis (B.A.) -- New College of Florida, 2014
- General Note:
- RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
- Bibliography:
- Includes bibliographical references.
- General Note:
- This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
- General Note:
- Faculty Sponsor: Beulig, Alfred
- Statement of Responsibility:
- by Alexandra Quezada
Record Information
- Source Institution:
- New College of Florida
- Holding Location:
- New College of Florida
- Rights Management:
- Applicable rights reserved.
- Classification:
- S.T. 2014 Q4
- System ID:
- AA00024793:00001
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