Material Information
- Title:
- CIRCADIAN REGULATION OF OXIDATIVE FAT METABOLISM IN C2C12 MYOTUBES
- Physical Description:
- Book
- Language:
- English
- Creator:
- Bacsik, David
- Publisher:
- New College of Florida
- Place of Publication:
- Sarasota, Fla.
- Publication Date:
- 2014
Thesis/Dissertation Information
- Degree:
- Bachelor's ( B.A.)
- Degree Grantor:
- New College of Florida
- Degree Divisions:
- Natural Sciences
- Area of Concentration:
- Biology
Subjects
- Genre:
- bibliography ( marcgt )
theses ( marcgt )
government publication (state, provincial, territorial, dependent) ( marcgt )
born-digital ( sobekcm )
Electronic Thesis or Dissertation
Notes
- Abstract:
- The molecular circadian clock consists of a transcription-translation feedback loop that
drives the rhythmic expression of hundreds of genes. The rate-limiting enzyme of the
NAD+ salvage pathway, nicotinamide phosphoribosyltransferase (NAMPT), is a known
target of the molecular circadian clock. Recently, the Bass Lab group at Northwestern
University elucidated an NAD+ dependent pathway connecting the molecular circadian
clock to fatty acid oxidation in mouse liver. Circadian regulation of oxidative fat
metabolism coordinates metabolic activities with circadian feeding and fasting
behaviors. To facilitate further study of this pathway, a mouse skeletal muscle cell line,
C2C12, was tested as a model system for studying fat metabolism and circadian clock
function. Rhythms of NAD+ concentration and mitochondrial respiration were observed
in synchronized C2C12 myotubes. NAMPT was found to have substantial influence on
cellular NAD+ concentrations, however, NAMPT expression as measured by qPCR
varied little throughout the day. These data suggest that, although further investigation
into the regulation of NAMPT activity is required, the C2C12 cell line may be a useful
model for the study of circadian metabolism.
- Statement of Responsibility:
- by David Bacsik
- Thesis:
- Thesis (B.A.) -- New College of Florida, 2014
- General Note:
- Online version not currently available.
- General Note:
- RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
- Bibliography:
- Includes bibliographical references.
- General Note:
- This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
- General Note:
- Faculty Sponsor: Clore, Amy
Record Information
- Source Institution:
- New College of Florida
- Holding Location:
- New College of Florida
- Rights Management:
- Applicable rights reserved.
- Classification:
- S.T. 2014 B33
- System ID:
- AA00024708:00001
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