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PURIFICATION AND CHARACTERIZATION OF ACETYLCHOLINESTERASE FROM DROSOPHILA SUZUKII

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004879/00001

Material Information

Title: PURIFICATION AND CHARACTERIZATION OF ACETYLCHOLINESTERASE FROM DROSOPHILA SUZUKII
Physical Description: Book
Language: English
Creator: Valbuena, Valeria
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2013
Publication Date: 2013

Subjects

Subjects / Keywords: Drosophila
Acetylcholinesterase
Purification
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Drosophila suzukii (Matsumura) is an invasive fly species and a current agricultural threat to soft peel fruit, such as strawberries and cherries, in the United States. Native to South East Asia, D. suzukii females have a serrated ovipositor that enables them to pierce the skin of ripening fruit to lay their eggs inside, which leads to the eventual decay of the fruit. D. suzukii has been exposed to pesticides throughout Asia, which may have conferred resistance via a mutated form of the enzyme acetylcholinesterase (AChE). AChE carries out a key step in the termination of synaptic transmission by hydrolyzing the neurotransmitter acetylcholine. AChE is the target of many current pesticides because its inhibition leads to ataxia, convulsions, and eventual death in the insects. In this study, AChE was purified from the heads of 5 day old D. suzukii flies by sequential extraction procedures followed by size exclusion chromatography. The partially purified AChE was used to optimize the pH and temperature of the reaction. Protein concentration assays were completed to calculate specific activity, and kinetic assays were performed. A Km value of 27 +/- 2 μM and a specific activity of 0.23 units/mg of protein were determined.
Statement of Responsibility: by Valeria Valbuena
Thesis: Thesis (B.A.) -- New College of Florida, 2013
General Note: Online version not currently available.
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine; McCord, Elzie

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2013 V1
System ID: NCFE004879:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004879/00001

Material Information

Title: PURIFICATION AND CHARACTERIZATION OF ACETYLCHOLINESTERASE FROM DROSOPHILA SUZUKII
Physical Description: Book
Language: English
Creator: Valbuena, Valeria
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2013
Publication Date: 2013

Subjects

Subjects / Keywords: Drosophila
Acetylcholinesterase
Purification
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Drosophila suzukii (Matsumura) is an invasive fly species and a current agricultural threat to soft peel fruit, such as strawberries and cherries, in the United States. Native to South East Asia, D. suzukii females have a serrated ovipositor that enables them to pierce the skin of ripening fruit to lay their eggs inside, which leads to the eventual decay of the fruit. D. suzukii has been exposed to pesticides throughout Asia, which may have conferred resistance via a mutated form of the enzyme acetylcholinesterase (AChE). AChE carries out a key step in the termination of synaptic transmission by hydrolyzing the neurotransmitter acetylcholine. AChE is the target of many current pesticides because its inhibition leads to ataxia, convulsions, and eventual death in the insects. In this study, AChE was purified from the heads of 5 day old D. suzukii flies by sequential extraction procedures followed by size exclusion chromatography. The partially purified AChE was used to optimize the pH and temperature of the reaction. Protein concentration assays were completed to calculate specific activity, and kinetic assays were performed. A Km value of 27 +/- 2 μM and a specific activity of 0.23 units/mg of protein were determined.
Statement of Responsibility: by Valeria Valbuena
Thesis: Thesis (B.A.) -- New College of Florida, 2013
General Note: Online version not currently available.
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine; McCord, Elzie

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2013 V1
System ID: NCFE004879:00001

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