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THE WORM, THE MYTH, THE LEGEND

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004814/00001

Material Information

Title: THE WORM, THE MYTH, THE LEGEND QUANTIFICATION OF MALE rha-1 MUTANT GERMLINE GENE EXPRESSION REGULATED BY SMALL RNA PATHWAYS OF Caenorhabditis elegans
Physical Description: Book
Language: English
Creator: Lindsay, Penelope
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2013
Publication Date: 2013

Subjects

Subjects / Keywords: C. Elegans
RNA Interference
qRT-PCR
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: RNA Helicase A (RHA-1) is a conserved protein thought to be involved in the regulation of germline gene expression in C. elegans. Previous studies involving mutants lacking RHA-1 exhibited a temperature sensitive sterility defect similar to eri-1 mutants. ERI-1 is an extensively studied protein known to have a key role in germline gene regulation through RNA interference, wherein small segments of RNA suppress gene expression. Since ERI-1 has a regulatory role in 26G RNAi, and eri-1 mutants exhibit a similar phenotype as rha-1 mutants, it was postulated that RHA-1 may also have a role in the eri-1-dependent RNAi pathway. RHA-1 appears to affect spermatogenesis in particular, so male him-8 mutants were used to allow for an examination of worms that produce sperm for a longer period of time. Through the use of quantitative real-time RT-PCR (qRT-PCR), several genes thought to be regulated by the 26G and 21U RNAi pathways were tested in mutant strains lacking RHA-1, ERI-1, or both RHA-1 and ERI-1. It was found that rha-1 mutants do not have significantly altered gene expression of the genes tested that are regulated by the 26G pathway. Although RHA-1 does not appear to significantly affect gene expression levels tested, there is still evidence that it plays a regulatory role in spermatogenesis-related processes, given previous phenotype observations. There is still a possibility that it may be involved in alternate RNAi pathways.
Statement of Responsibility: by Penelope Lindsay
Thesis: Thesis (B.A.) -- New College of Florida, 2013
General Note: Online version not currently available.
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2013 L7
System ID: NCFE004814:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004814/00001

Material Information

Title: THE WORM, THE MYTH, THE LEGEND QUANTIFICATION OF MALE rha-1 MUTANT GERMLINE GENE EXPRESSION REGULATED BY SMALL RNA PATHWAYS OF Caenorhabditis elegans
Physical Description: Book
Language: English
Creator: Lindsay, Penelope
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2013
Publication Date: 2013

Subjects

Subjects / Keywords: C. Elegans
RNA Interference
qRT-PCR
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: RNA Helicase A (RHA-1) is a conserved protein thought to be involved in the regulation of germline gene expression in C. elegans. Previous studies involving mutants lacking RHA-1 exhibited a temperature sensitive sterility defect similar to eri-1 mutants. ERI-1 is an extensively studied protein known to have a key role in germline gene regulation through RNA interference, wherein small segments of RNA suppress gene expression. Since ERI-1 has a regulatory role in 26G RNAi, and eri-1 mutants exhibit a similar phenotype as rha-1 mutants, it was postulated that RHA-1 may also have a role in the eri-1-dependent RNAi pathway. RHA-1 appears to affect spermatogenesis in particular, so male him-8 mutants were used to allow for an examination of worms that produce sperm for a longer period of time. Through the use of quantitative real-time RT-PCR (qRT-PCR), several genes thought to be regulated by the 26G and 21U RNAi pathways were tested in mutant strains lacking RHA-1, ERI-1, or both RHA-1 and ERI-1. It was found that rha-1 mutants do not have significantly altered gene expression of the genes tested that are regulated by the 26G pathway. Although RHA-1 does not appear to significantly affect gene expression levels tested, there is still evidence that it plays a regulatory role in spermatogenesis-related processes, given previous phenotype observations. There is still a possibility that it may be involved in alternate RNAi pathways.
Statement of Responsibility: by Penelope Lindsay
Thesis: Thesis (B.A.) -- New College of Florida, 2013
General Note: Online version not currently available.
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2013 L7
System ID: NCFE004814:00001

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