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THE POSSIBLE ROLE OF rha-1 IN THE piRNA GENE REGULATORY PATHWAYS IN THE GERMLINE OF Caenorhabditis elegans

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004764/00001

Material Information

Title: THE POSSIBLE ROLE OF rha-1 IN THE piRNA GENE REGULATORY PATHWAYS IN THE GERMLINE OF Caenorhabditis elegans
Physical Description: Book
Language: English
Creator: Gautier, Megan
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2013
Publication Date: 2013

Subjects

Subjects / Keywords: C. Elegans
piRNA
rha-1
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: RNA Helicase A (RHA-1) is a highly conserved protein thought to be involved in the regulation of gene expression within the germline of C. elegans. Previous studies found that mutant nematodes lacking RHA-1 had a temperature sensitive sterility defect similar to those found in mut-7 and rde-2 mutants. The MUT-7 and RDE-2 proteins are known to be involved in 22G-RNA interference, a gene regulation pathway wherein short segments of RNA inhibit the expression of specific target genes. Due to the similar phenotypes noted between these three mutants, it was hypothesized that RHA-1 may also function within the 22G-RNAi pathway, which acts downstream of the recently discovered 21U-piRNA pathway. Double mutants lacking either both RDE-2 and RHA-1 or MUT-7 and RHA-1 were created to explore the effects on the hermaphrodite germline. Preliminary quantification of piRNA-regulated transposons utilizing qRT-PCR revealed that rha-1 mutants did not overexpress the transposons, but RHA-1 activity may be required for the overexpression observed in rde-2 and mut-7 mutants. Through the use of mating assays and microscopy, it was found that when the rha-1 and mut-7 mutations are combined, a previously reported sperm defect within the mut-7 strain is exacerbated such that severe defects in spermatogenesis are observable at lower temperatures. It is thus proposed that RHA-1 may function within regulation of either the initiation of sperm meiosis or in the switch from sperm to oocyte production within the developing hermaphrodite germline.
Statement of Responsibility: by Megan Gautier
Thesis: Thesis (B.A.) -- New College of Florida, 2013
General Note: Online version not currently available.
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2013 G2
System ID: NCFE004764:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004764/00001

Material Information

Title: THE POSSIBLE ROLE OF rha-1 IN THE piRNA GENE REGULATORY PATHWAYS IN THE GERMLINE OF Caenorhabditis elegans
Physical Description: Book
Language: English
Creator: Gautier, Megan
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2013
Publication Date: 2013

Subjects

Subjects / Keywords: C. Elegans
piRNA
rha-1
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: RNA Helicase A (RHA-1) is a highly conserved protein thought to be involved in the regulation of gene expression within the germline of C. elegans. Previous studies found that mutant nematodes lacking RHA-1 had a temperature sensitive sterility defect similar to those found in mut-7 and rde-2 mutants. The MUT-7 and RDE-2 proteins are known to be involved in 22G-RNA interference, a gene regulation pathway wherein short segments of RNA inhibit the expression of specific target genes. Due to the similar phenotypes noted between these three mutants, it was hypothesized that RHA-1 may also function within the 22G-RNAi pathway, which acts downstream of the recently discovered 21U-piRNA pathway. Double mutants lacking either both RDE-2 and RHA-1 or MUT-7 and RHA-1 were created to explore the effects on the hermaphrodite germline. Preliminary quantification of piRNA-regulated transposons utilizing qRT-PCR revealed that rha-1 mutants did not overexpress the transposons, but RHA-1 activity may be required for the overexpression observed in rde-2 and mut-7 mutants. Through the use of mating assays and microscopy, it was found that when the rha-1 and mut-7 mutations are combined, a previously reported sperm defect within the mut-7 strain is exacerbated such that severe defects in spermatogenesis are observable at lower temperatures. It is thus proposed that RHA-1 may function within regulation of either the initiation of sperm meiosis or in the switch from sperm to oocyte production within the developing hermaphrodite germline.
Statement of Responsibility: by Megan Gautier
Thesis: Thesis (B.A.) -- New College of Florida, 2013
General Note: Online version not currently available.
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2013 G2
System ID: NCFE004764:00001

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