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Phenotypic Descriptions of Caenorhabditis elegans Mutants and Double Mutants

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004415/00001

Material Information

Title: Phenotypic Descriptions of Caenorhabditis elegans Mutants and Double Mutants
Physical Description: Book
Language: English
Creator: Muschler, Karen
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2011
Publication Date: 2011

Subjects

Subjects / Keywords: C.elegans
Genetics
rha-7
Nucroscopy
Fluorensence
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Caenorhabditis elegans is a convenient genetic model organism. A deletion in one of the many proteins in a small-RNA mediated gene silencing pathway, rha-1, was previously found to cause sterility at restrictive temperatures (25�C) and meiotic abnormalities. To further study rha-1 in C. elegans, this mutant was compared to a phenotypically similar strain with a mutation in eri-1. A double mutant (rha-1;eri-1) was used to study the relationship between the genes and their roles in gametogenesis. Mating assays with both the males and hermaphrodites were conducted at 20�C and 25�C to compare fertility in the mutants. Dissections were made of the male and hermaphrodite gonads and were treated with anti-pH3S10 antibody, then stained with AlexaFluor Green 488, a fluorescent green dye. This antibody binds to mitotic nuclei in the distal gonad and late meiotic nuclei (both oocytes and spermatocytes) in the proximal gonad. It was found that at 20�C, rha-1 males and hermaphrodites reproduce sufficiently. At 25�C, however, the rha-1 hermaphrodites produced fewer offspring than wild-type worms, and the males were 83% sterile. The fertility of the double mutant was very similar to the eri-1 mutants of both sexes at both temperatures, but the double mutant hermaphrodites produced fewer offspring at 25�C than either single mutant strain. Dissections of the hermaphrodites revealed that the oocytes may show some strain-specific defects but differences were not able to be statistically analyzed.
Statement of Responsibility: by Karen Muschler
Thesis: Thesis (B.A.) -- New College of Florida, 2011
General Note: Online version not currently available.
Supplements: Accompanying materials: CD
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2011 M98
System ID: NCFE004415:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004415/00001

Material Information

Title: Phenotypic Descriptions of Caenorhabditis elegans Mutants and Double Mutants
Physical Description: Book
Language: English
Creator: Muschler, Karen
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2011
Publication Date: 2011

Subjects

Subjects / Keywords: C.elegans
Genetics
rha-7
Nucroscopy
Fluorensence
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Caenorhabditis elegans is a convenient genetic model organism. A deletion in one of the many proteins in a small-RNA mediated gene silencing pathway, rha-1, was previously found to cause sterility at restrictive temperatures (25�C) and meiotic abnormalities. To further study rha-1 in C. elegans, this mutant was compared to a phenotypically similar strain with a mutation in eri-1. A double mutant (rha-1;eri-1) was used to study the relationship between the genes and their roles in gametogenesis. Mating assays with both the males and hermaphrodites were conducted at 20�C and 25�C to compare fertility in the mutants. Dissections were made of the male and hermaphrodite gonads and were treated with anti-pH3S10 antibody, then stained with AlexaFluor Green 488, a fluorescent green dye. This antibody binds to mitotic nuclei in the distal gonad and late meiotic nuclei (both oocytes and spermatocytes) in the proximal gonad. It was found that at 20�C, rha-1 males and hermaphrodites reproduce sufficiently. At 25�C, however, the rha-1 hermaphrodites produced fewer offspring than wild-type worms, and the males were 83% sterile. The fertility of the double mutant was very similar to the eri-1 mutants of both sexes at both temperatures, but the double mutant hermaphrodites produced fewer offspring at 25�C than either single mutant strain. Dissections of the hermaphrodites revealed that the oocytes may show some strain-specific defects but differences were not able to be statistically analyzed.
Statement of Responsibility: by Karen Muschler
Thesis: Thesis (B.A.) -- New College of Florida, 2011
General Note: Online version not currently available.
Supplements: Accompanying materials: CD
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2011 M98
System ID: NCFE004415:00001

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