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Cellular and Genetic Factors in Traumatic Brain Injury

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004096/00001

Material Information

Title: Cellular and Genetic Factors in Traumatic Brain Injury A Histological Analysis
Physical Description: Book
Language: English
Creator: Ganey, Christopher
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2009
Publication Date: 2009

Subjects

Subjects / Keywords: Biology
Neurobiology
Brain Injury
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Traumatic brain is a growing concern among the general population with debilitating long -term effects. The introduction in the thesis will review other literature on traumatic brain injury to give the reader a brief overview of the topic. I hypothesized that traumatic brain injury has a profound effect on physiological activity. I also hypothesized that there is genetic influence on the outcome of injury as well. I performed histological analyses on brain tissues of mice that have undergone traumatic brain injury experiments at the Roskamp Institute. The results from my analyses have indicated that both traumatic brain injury and genetic factors do have a substantial effect on the overall outcome.
Statement of Responsibility: by Christopher Ganey
Thesis: Thesis (B.A.) -- New College of Florida, 2009
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Demski, Leo

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2009 G1
System ID: NCFE004096:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004096/00001

Material Information

Title: Cellular and Genetic Factors in Traumatic Brain Injury A Histological Analysis
Physical Description: Book
Language: English
Creator: Ganey, Christopher
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2009
Publication Date: 2009

Subjects

Subjects / Keywords: Biology
Neurobiology
Brain Injury
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Traumatic brain is a growing concern among the general population with debilitating long -term effects. The introduction in the thesis will review other literature on traumatic brain injury to give the reader a brief overview of the topic. I hypothesized that traumatic brain injury has a profound effect on physiological activity. I also hypothesized that there is genetic influence on the outcome of injury as well. I performed histological analyses on brain tissues of mice that have undergone traumatic brain injury experiments at the Roskamp Institute. The results from my analyses have indicated that both traumatic brain injury and genetic factors do have a substantial effect on the overall outcome.
Statement of Responsibility: by Christopher Ganey
Thesis: Thesis (B.A.) -- New College of Florida, 2009
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Demski, Leo

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2009 G1
System ID: NCFE004096:00001


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Full Text

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Acknowledgments

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Table of Contents 1. Title i 2. Acknowledgements ...ii 3. Table of Contents.iii 4. List of Figures and Graphs ............................v 5. Abstract vii 6. Introduction .1 6.1 Introduction to Traumatic Brain Injury..1 6.2 Microglias Function and role in TBI .... 6.3 Astrocytes Function and role in TBI.....7 6.4 ApoE and its relation to TBI.8 7. Methods and Procedures.38 7.1 Introduction .38 7.2 Experiments 7.2a Apo e 133 149 .38 7.2b APP vs. WT 39 7.2c 2 vs. 4 .. 39 7.2d TBI Severity Study.......40 7.3 General Overview .40

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7.3a TBI infliction.. 40 7.3b Fixation, Embedding and Sectioning 7.3c Staining Techniques .41 7.4d Quantification and Statistical Analysis ..43 8. Results...44 8.1 ApoE 133 149 44 8.1a Microglia Analysis ...44 8.1b Astrocyte Analysis ..45 8.2 APP vs. WT 8.2a 4G8 Analysis 8.2b Astrocyte Analysis .....47 8.3 2 vs. 4 ..47 8.3a Microglia Analysis ...48 8.3b Astro cyte Analysis ..49 8.4 TBI Severity Study ..49 8.4a Astrocyte Analysis ..49 9. Conclusion..52 10. Bibliography.. 54

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List of Figures and Graphs Figures Figure 1 .1 Figure 2 .6 Figure 3 ..10 Figure 4 ..12 Figure 5 ..13 Figure 6 ..15 Figure 7 ..16 Figure 8 ..18 Figure 9 ..19 Figure 10 Figure 11 Figure 12 Figure 13 Figure 14 Figure 15 Figure 16 Fig ure 17

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Figure 18 Figure 19 Figure 20 Figure 21 Graphs Graph 1 ...44 Graph 2 ...45 Graph 3 ...46 Graph 4 ...47 Graph 5 ...48 Graph 6 ...49 Graph 7 ...50

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1. Introduction 1.1 Introduction to Traumatic Brain Injury Figure 1 Figure 1.

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Figure 2.

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Figure 2. a c d e d e f g f g 1.2 Microglias functions and role in traumatic brain injury

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1.3 Astrocytes function and role in traumatic brain injury

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1.4 ApoE and its relation to TBI

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Figure 3 Figure 3. a) b) c) d) Figure 3d

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p p p Figure 4

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Figure 4. Figure 5

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Figure 5. A) B) C) D)

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p p

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Fig. 6 Figure 7. Figure 6.

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Figure 7. A) B) C) p

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Figure 8 p < p

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Figure 8 p t in vivo Figure 9

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in vitro Figure 9. in vivo Figure 10 in vivo in vitro

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F igure 10. In vivo

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p Figure 11a Figure 11b

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Figure 11. a) b) p<

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Figure 12 Figure 12. p

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Figure 13a Figure 13b Figure 14

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Figure 13. a) p b) Figure 14

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Figure 15. Figure 15. A) B) p

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p p Figure 16

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Figure 16.

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Figure 17 Figure 18 NH CH3CH2O-O OH O Figure 17. Figure 18.

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p p<. Figure 19

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Figure 19. p Figure 20

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a) b) Figure 20. a) b)

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dementia pugilistica Figure 21

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Figure 21.

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2. Methods and Procedures2.1 Introduction 2.2 xperiments E2.2a ApoE 133 149

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2.2b APPvs. WT 2.2c. 2 vs. 4

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2.2d Traumatic Brain Injury Severity Study 2.3 General Overview 2.3.a TBIInfliction 2.3b Fixation, Embedding and Sectioning

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2.3c Staining Techniques

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2.4d Quantification and Statistical Analysis P

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3. Results 3.1 ApoE 133 149 3.1a Microglial Analysis Graph 1. Graph1. P p p

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3.1b Astrocyteanalysis Graph 2. Graph 2. P ShamVehicle Cortex vs TBI Vehicle Cortex p <0.01 ShamVehicle Cortex vs TBI Vehicle Hippocampus p <0.001 ShamVehicle Thalamus vs TBI Vehicle Cortex p <0.01 ShamVehicle Thalamus vs TBI Vehicle Hippocampus p <0.001 ShamVehicle Hippocampus vs TBI Vehicle Cortex p <0.01

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ShamVehicle Hippocampus vs TBI Vehicle Hip p <0. 001 ShamApoE Cortex vs TBI Vehicle Cortex p <0.01 ShamApoE Cortex vs TBI Vehicle Hippocampus p <0.001 ShamApoE Thalamus vs TBI Vehicle Cortex p <0.01 ShamApoE Thalamus vs TBI Vehicle Hippocampus p <0.001 ShamApoE Hippocampus vs TBI Vehicle Cortex p <0.05 SgamApoE Hippocampus vs TBI Vehicle Hippocampus p <0.001 TBI Vehicle Hippocampus vs TBI ApoE Cortex p <0.05 TBI Vehicle Hippocampus vs TBI ApoE Thalamus p <0.01 TBI Vehicle Hippocampus vs TBI ApoE Hippocampus p <0.01 3.2APP vs. WT 3.2a 4G8 positive plaquecount Graph 3. Graph 3 P

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3 .2b Astroc ytelevels b) c) Graph 4. a) b) c) P p 3 3 .3 2 vs. 4.3a Micro glial analysis

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a) b)Graph 5. a) b) Graphs 5a 5b P p

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3 .3b GFAP anal ysis Graph 6. Graph 6 P p 3.4Traumatic Br ain Injury Severity Study 3.4a Astrocyte 2 weekand 4 weekanalysis

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a) b) Graph 7. a) b) Graphs 7 P

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2week Sham Cortex vs 4week TBI 1.3mm Cortex P<0.01 2week Sham Cortex vs 4week TBI 2.0mm Cortex P<0.05 2week Sham Hippocampus vs 4week TBI 1.3mm Cortex P<0.05 2week Sham Hippocampus vs 4week TBI 2.0mm Cortex P<0.05 2week TBI 1.3mm Hippocampus vs 4week TBI 1.3mm Cortex P<0.05 4week TBI 1.3mm Cortex vs 4week Sham Hippocampus P<0.01 4week TBI 1.3mm Cortex vs 4week TBI 1.3mm Hippocampus P<0.01 4week TBI 1.3mm Cortex vs 4week TBI 2.0mm Hippocampus P<0.05 4week TBI 2.0 mm Cortex vs 4week Sham Hippocampus P<0.05 4week TBI 2.0mm Cortex vs 4week TBI 1.3mm Hippocampus P<0.05 4week TBI 2.0mm Cortex vs 4week TBI 2.0 mm Hippocampus P<0.05

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4. Conclusion

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5. Bibliography

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T. J. (2006). Neurotoxicity from innate immune response is greatest with targeted replacement of E4 allele of apolipoprotein E gene and is mediated by microglial p38MAPK. The FASEB Journal, 20(6) Marciano, P. G., Brettschneider, J., Manduchi, E., Davis, J. E., Eastman, S., Raghupathi, R., et al. (2004). Neuron-specific mRNA complexity responses duri ng hippocampal apoptosis after traumatic brain injury. The Journal of Neuroscience, 24 (12), 2866-2876. McAdoo, J. D., Warner, D. S., Goldberg, R. N., Vitek, M. P., Pearlstein, R., & Laskowitz, D. T. (2005). Intrathecal administration of a novel apoE-derived therapeutic peptide improves outcome following peri natal hypoxi-ischemic injury. Neuroscience Letters, 381 305-308. Mey, J. (2006). New therapeutic ta rget for CNS injury? the role of retinoic acid signa ling after nerve lesions. Journal of Neurobiology, 66 (7), 757-779. Ming, G., & Song, H. (2005). Adult neurogenesis in the mammalian central nervous system. Annual Review of Neuroscience, 28 223-250. Morganti-Kossmann, M. C., Satgunaseelan, L., Bye, N., & Kossmann, T. (2007). Modulation of immune response by head injury. Injury International Journal of the Care of the Injured, 38 1392-1400.

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