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Toxicological Effects of Brevetoxin-2 (PbTx-2) on the Fall Armyworm, Spodoptera frugiperda (J.E. Smith)

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004021/00001

Material Information

Title: Toxicological Effects of Brevetoxin-2 (PbTx-2) on the Fall Armyworm, Spodoptera frugiperda (J.E. Smith)
Physical Description: Book
Language: English
Creator: Silpa, Marc Andrew Charles
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2008
Publication Date: 2008

Subjects

Subjects / Keywords: Brevetoxin
PbTx-2
Brevetoxin-2
Spodoptera frugiperda
Fall Armyworm
Red Tide
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Brevetoxin (PbTx), the primary neurotoxin of Red Tide, was tested in vivo on the fall annyworm to determine if a lethal dose could be established. Ten fourth instar larvae per treatment, replicated six times, were fed nanomolar concentrations of PbTx-2 on dipped cabbage leaves for 72 hours. High cannibalism levels were observed facilitating a repeat of this experiment. Five additional higher (micromolar) PbTx-2 concentrations were included in the subsequent study as well as a deposit on diet assay was performed. The deposit on diet assay involved placing approximately 1-2 g hot meridic diet in 1 oz. plastic creamers. Two hundred microliters (200I1L) of brevet ox in formulation was pipetted atop diet in each cup, corresponding to rate and consisting of three replicates of 10 larvae per treatment. LC50 was not obtained because mortality did not exceed 10% in either test. In vivo observations did not produce discernible evidence of toxicity; however, other observation showed variable adult emergence even though only a weak correlation could be established. Future experiments should include higher in vivo doses and in vitro detoxification and enzyme inhibition/induction assays to elucidate the effects of brevetoxin on this species.
Statement of Responsibility: by Marc Andrew Charles Silpa
Thesis: Thesis (B.A.) -- New College of Florida, 2008
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: McCord, Elzie

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2008 S58
System ID: NCFE004021:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE004021/00001

Material Information

Title: Toxicological Effects of Brevetoxin-2 (PbTx-2) on the Fall Armyworm, Spodoptera frugiperda (J.E. Smith)
Physical Description: Book
Language: English
Creator: Silpa, Marc Andrew Charles
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2008
Publication Date: 2008

Subjects

Subjects / Keywords: Brevetoxin
PbTx-2
Brevetoxin-2
Spodoptera frugiperda
Fall Armyworm
Red Tide
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Brevetoxin (PbTx), the primary neurotoxin of Red Tide, was tested in vivo on the fall annyworm to determine if a lethal dose could be established. Ten fourth instar larvae per treatment, replicated six times, were fed nanomolar concentrations of PbTx-2 on dipped cabbage leaves for 72 hours. High cannibalism levels were observed facilitating a repeat of this experiment. Five additional higher (micromolar) PbTx-2 concentrations were included in the subsequent study as well as a deposit on diet assay was performed. The deposit on diet assay involved placing approximately 1-2 g hot meridic diet in 1 oz. plastic creamers. Two hundred microliters (200I1L) of brevet ox in formulation was pipetted atop diet in each cup, corresponding to rate and consisting of three replicates of 10 larvae per treatment. LC50 was not obtained because mortality did not exceed 10% in either test. In vivo observations did not produce discernible evidence of toxicity; however, other observation showed variable adult emergence even though only a weak correlation could be established. Future experiments should include higher in vivo doses and in vitro detoxification and enzyme inhibition/induction assays to elucidate the effects of brevetoxin on this species.
Statement of Responsibility: by Marc Andrew Charles Silpa
Thesis: Thesis (B.A.) -- New College of Florida, 2008
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: McCord, Elzie

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2008 S58
System ID: NCFE004021:00001

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