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Use of Vitellogenin as a Biomarker for Ecoestrogen Exposure in Hogchockers (Trinectes maculatus) from the Caloosahatchee...

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003880/00001

Material Information

Title: Use of Vitellogenin as a Biomarker for Ecoestrogen Exposure in Hogchockers (Trinectes maculatus) from the Caloosahatchee and Myakka Rivers
Physical Description: Book
Language: English
Creator: Arnold, Mariah
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2008
Publication Date: 2008

Subjects

Subjects / Keywords: Estrogen
Biomarkers
Vitellogenin
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Ecoestrogens are synthetic and natural compounds capable of mimicking natural estrogens found in an organism. These compounds threaten the health of organisms living in aquatic ecosystems, so it is important to identify the types and sources of ecoestrogens present in the environment. The Caloosahatchee River receives wastewater effluent from multiple sources, while the Myakka River does not receive effluent. We used hogchokers (Trinectes maculatus) as an animal model to determine if ecoestrogens in the rivers were at bioactive concentrations. Hogchokers were collected from the Caloosahatchee (N=24) and Myakka (N=20) Rivers in Florida starting mid-September. Vitellogenin (Vtg), a female-specific egg yolk protein, was used as a biomarker for ecoestrogen exposure. Western blot staining and a quantitative ELISA were developed to analyze blood serum Vtg concentrations. A new phosphoprotein detection kit method was also tested. Data thus far show no abnormal expression of Vtg in Myakka and Caloosahatchee River hogchokers. Analysis of liver samples from the Caloosahatchee River hogchokers revealed the presence of foci of cellular alterations. These liver abnormalities indicate the presence of an as-yet unknown ecological stressor in the Caloosahatchee River. Research is continuing with more histological analysis of livers for the presence of other pollutant biomarkers.
Statement of Responsibility: by Mariah Arnold
Thesis: Thesis (B.A.) -- New College of Florida, 2008
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Beulig, Alfred

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2008 A7
System ID: NCFE003880:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003880/00001

Material Information

Title: Use of Vitellogenin as a Biomarker for Ecoestrogen Exposure in Hogchockers (Trinectes maculatus) from the Caloosahatchee and Myakka Rivers
Physical Description: Book
Language: English
Creator: Arnold, Mariah
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2008
Publication Date: 2008

Subjects

Subjects / Keywords: Estrogen
Biomarkers
Vitellogenin
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Ecoestrogens are synthetic and natural compounds capable of mimicking natural estrogens found in an organism. These compounds threaten the health of organisms living in aquatic ecosystems, so it is important to identify the types and sources of ecoestrogens present in the environment. The Caloosahatchee River receives wastewater effluent from multiple sources, while the Myakka River does not receive effluent. We used hogchokers (Trinectes maculatus) as an animal model to determine if ecoestrogens in the rivers were at bioactive concentrations. Hogchokers were collected from the Caloosahatchee (N=24) and Myakka (N=20) Rivers in Florida starting mid-September. Vitellogenin (Vtg), a female-specific egg yolk protein, was used as a biomarker for ecoestrogen exposure. Western blot staining and a quantitative ELISA were developed to analyze blood serum Vtg concentrations. A new phosphoprotein detection kit method was also tested. Data thus far show no abnormal expression of Vtg in Myakka and Caloosahatchee River hogchokers. Analysis of liver samples from the Caloosahatchee River hogchokers revealed the presence of foci of cellular alterations. These liver abnormalities indicate the presence of an as-yet unknown ecological stressor in the Caloosahatchee River. Research is continuing with more histological analysis of livers for the presence of other pollutant biomarkers.
Statement of Responsibility: by Mariah Arnold
Thesis: Thesis (B.A.) -- New College of Florida, 2008
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Beulig, Alfred

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2008 A7
System ID: NCFE003880:00001

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