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X-Linked Gene Expression in Wild-Type, rha-1(tm329), and rde-2(ne221) C. elegans Using Realt-Time Reverse Transcription ...

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003869/00001

Material Information

Title: X-Linked Gene Expression in Wild-Type, rha-1(tm329), and rde-2(ne221) C. elegans Using Realt-Time Reverse Transcription Polymerase Chain Reaction
Physical Description: Book
Language: English
Creator: White, Justin
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2007
Publication Date: 2007

Subjects

Subjects / Keywords: C. elegans
RHA-1
Real-Time RT PCR
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Helicases are enzymes that separate dsRNA or dsDNA and have been implicated in several different cellular processes. In particular, RNA helicase A (RHA) is a multifunctional helicase involved in transcription regulation and histone modification. A deletion in rha-1 in C. elegans produces a temperature sensitive sterile mutant (Walstrom et al., 2005). The mutant worms display a different series of histone modification and of particular interest is the loss of the histone 3 dimethylation on lysine 9. Rha- 1 also plays a role in the conserved RNA interference (RNAi) pathway, which is a post-transcriptional silencing pathway. Another gene required for RNAi is rde-2. We used a null mutant of rde-2 and the rha-1 mutant to determine the respective effects of the mutations on genetic expression of X-linked genes in the germline. Using Real-time RT-PCR, we discovered that there was little change the expression of many of the X-linked genes examined. There was more expression in the rha-1 mutants than in the rde- 2 mutants, which can be explained by the presence of the H3K9 methylation (silencing modification) in the rde-2 mutants but not in the rha-1 mutants. There seemed to be a defect in silencing with respect to gene T08D2.4 in both mutant strains. After examining the gene expression results, a double mutant was constructed.
Statement of Responsibility: by Justin White
Thesis: Thesis (B.A.) -- New College of Florida, 2007
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2007 W5
System ID: NCFE003869:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003869/00001

Material Information

Title: X-Linked Gene Expression in Wild-Type, rha-1(tm329), and rde-2(ne221) C. elegans Using Realt-Time Reverse Transcription Polymerase Chain Reaction
Physical Description: Book
Language: English
Creator: White, Justin
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2007
Publication Date: 2007

Subjects

Subjects / Keywords: C. elegans
RHA-1
Real-Time RT PCR
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Helicases are enzymes that separate dsRNA or dsDNA and have been implicated in several different cellular processes. In particular, RNA helicase A (RHA) is a multifunctional helicase involved in transcription regulation and histone modification. A deletion in rha-1 in C. elegans produces a temperature sensitive sterile mutant (Walstrom et al., 2005). The mutant worms display a different series of histone modification and of particular interest is the loss of the histone 3 dimethylation on lysine 9. Rha- 1 also plays a role in the conserved RNA interference (RNAi) pathway, which is a post-transcriptional silencing pathway. Another gene required for RNAi is rde-2. We used a null mutant of rde-2 and the rha-1 mutant to determine the respective effects of the mutations on genetic expression of X-linked genes in the germline. Using Real-time RT-PCR, we discovered that there was little change the expression of many of the X-linked genes examined. There was more expression in the rha-1 mutants than in the rde- 2 mutants, which can be explained by the presence of the H3K9 methylation (silencing modification) in the rde-2 mutants but not in the rha-1 mutants. There seemed to be a defect in silencing with respect to gene T08D2.4 in both mutant strains. After examining the gene expression results, a double mutant was constructed.
Statement of Responsibility: by Justin White
Thesis: Thesis (B.A.) -- New College of Florida, 2007
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2007 W5
System ID: NCFE003869:00001

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