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Sensitivity to dsRNA feeding, fertility, and X-Linked gene expression in wild type, rha-1(tm329), rde-2(ne211, rde-2;rha...

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003795/00001

Material Information

Title: Sensitivity to dsRNA feeding, fertility, and X-Linked gene expression in wild type, rha-1(tm329), rde-2(ne211, rde-2;rha-1, and mut-7;rha-1 mutant worms
Physical Description: Book
Language: English
Creator: Hughes, Amanda
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2007
Publication Date: 2007

Subjects

Subjects / Keywords: RNAi
Heterochromatin
Caenorhabditis elegans
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: The RNA helicase A protein in Caenorhabditis elegans (RHA-1) has been found to be involved in RNA interference (RNAi) (Robert et al., 2005), a method of post-transcriptional gene silencing, and in heterochromatin formation (Walstrom et al., 2005), a regulation of transcriptional gene silencing. RDE-2 and MUT-7, two proteins observed to interact with each other (Tops et al., 2005), are also involved in the RNAi response and in silencing of transposons (Tabara et al., 1999; Ketting et al., 1999). To determine any interactions between RHA-1, RDE-2 and MUT-7 and their involvement in the RNAi and related pathways, rde-2;rha-1 and mut-7;rha-1 double mutants were observed for responsiveness to RNAi, X-linked gene expression, and fertility. RHA-1 was found to genetically interact with RDE- 2 and MUT-7 differently in all three of these areas. X-linked gene expression of the genes T08D2.3 and T08D2.4 was increased similarly in rde-2;rha-1 and rha-1(tm329) strains as compared to wild type, but was not in mut-7;rha-1 mutants. Fertility was increased in the rde-2;rha-1 double mutant over the single mutants, although still decreased as compared to wild type, but mut-7;rha-1 experienced a more severe infertile phenotype. rha-1(tm329) mutation increased somatic RNAi sensitivity when alone or in combination with an rde-2(ne211) mutation; however, the mut-7;rha-1 double mutant had decreased sensitivity to somatic RNAi as compared to wild type. RDE-2 and MUT-7 appear to have different functions in somatic RNAi, despite their physical interaction. These observations might imply that RDE-2 and MUT-7 act in opposition to one another, or have distinct roles in RNAi.
Statement of Responsibility: by Amanda Hughes
Thesis: Thesis (B.A.) -- New College of Florida, 2007
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2007 H89
System ID: NCFE003795:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003795/00001

Material Information

Title: Sensitivity to dsRNA feeding, fertility, and X-Linked gene expression in wild type, rha-1(tm329), rde-2(ne211, rde-2;rha-1, and mut-7;rha-1 mutant worms
Physical Description: Book
Language: English
Creator: Hughes, Amanda
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2007
Publication Date: 2007

Subjects

Subjects / Keywords: RNAi
Heterochromatin
Caenorhabditis elegans
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: The RNA helicase A protein in Caenorhabditis elegans (RHA-1) has been found to be involved in RNA interference (RNAi) (Robert et al., 2005), a method of post-transcriptional gene silencing, and in heterochromatin formation (Walstrom et al., 2005), a regulation of transcriptional gene silencing. RDE-2 and MUT-7, two proteins observed to interact with each other (Tops et al., 2005), are also involved in the RNAi response and in silencing of transposons (Tabara et al., 1999; Ketting et al., 1999). To determine any interactions between RHA-1, RDE-2 and MUT-7 and their involvement in the RNAi and related pathways, rde-2;rha-1 and mut-7;rha-1 double mutants were observed for responsiveness to RNAi, X-linked gene expression, and fertility. RHA-1 was found to genetically interact with RDE- 2 and MUT-7 differently in all three of these areas. X-linked gene expression of the genes T08D2.3 and T08D2.4 was increased similarly in rde-2;rha-1 and rha-1(tm329) strains as compared to wild type, but was not in mut-7;rha-1 mutants. Fertility was increased in the rde-2;rha-1 double mutant over the single mutants, although still decreased as compared to wild type, but mut-7;rha-1 experienced a more severe infertile phenotype. rha-1(tm329) mutation increased somatic RNAi sensitivity when alone or in combination with an rde-2(ne211) mutation; however, the mut-7;rha-1 double mutant had decreased sensitivity to somatic RNAi as compared to wild type. RDE-2 and MUT-7 appear to have different functions in somatic RNAi, despite their physical interaction. These observations might imply that RDE-2 and MUT-7 act in opposition to one another, or have distinct roles in RNAi.
Statement of Responsibility: by Amanda Hughes
Thesis: Thesis (B.A.) -- New College of Florida, 2007
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2007 H89
System ID: NCFE003795:00001

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