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Kinetics of IRP1 Gene Expression in Gravistimulated Maize Pulvini

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003761/00001

Material Information

Title: Kinetics of IRP1 Gene Expression in Gravistimulated Maize Pulvini
Physical Description: Book
Language: English
Creator: Doore, Sarah M.
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2007
Publication Date: 2007

Subjects

Subjects / Keywords: Plant Cell Biology
Cellular Signaling
Gravitropism
Gravistimulation
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: During a plant's lifespan, it is bound to encounter and respond to many stimuli, including changes in orientation with respect to the gravity vector. During harsh storms or gusts of wind, a plant may tip over, and it is necessary for it to right itself in order to continue reasonably normal growth. Many monocots achieve this reorientation by differential growth in specialized discs of tissue along the stem known as pulvini. Following stimulation, the cells in the lower half of the pulvini elongate, leading to an upward curvature of the stem. Gravity perception occurs in specialized cells known as statocytes, which contain amyloplasts that sediment upon reorientation. How this sedimentation leads to a change in growth is still largely unknown. Although recent studies have begun tackling the pieces of the signaling pathway, little is understood about the changes in gene expression that lead to differential growth. Preliminary results of Clore et al. (in preparation) suggest that the iron regulatory protein 1 (irp1) gene is upregulated soon after gravistimulation in maize (Zea mays), and that IRP1 may play a part in the early signaling pathway prior to differential growth. We aimed to further test this hypothesis by measuring the levels of irp1 mRNA at various timepoints after gravistimulation in both upper and lower halves of the pulvinus. To do so, we used the sensitive and quantitative technique of real time reverse-transcriptase polymerase chain reaction (real-time RT-PCR). We have determined that there appears to be a pattern of synchronous rises and falls of irp1 transcript levels in both the upper and lower halves of the pulvinus, whereas gene expression is reasonably steady in vertical pulvini and in gravistimulated internode tissue. Somewhat unexpectedly, modest increases in expression did occur in node tissues as well as leaf sheath tissue immediately around the pulvinus. Possible explanations for this result are discussed. We hypothesize that IRP1 may be part of a cascade that leads from gravity perception to asymmetrical growth and propose future experiments to study this possibility further.
Statement of Responsibility: by Sarah M. Doore
Thesis: Thesis (B.A.) -- New College of Florida, 2007
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Clore, Amy

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2007 D69
System ID: NCFE003761:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003761/00001

Material Information

Title: Kinetics of IRP1 Gene Expression in Gravistimulated Maize Pulvini
Physical Description: Book
Language: English
Creator: Doore, Sarah M.
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2007
Publication Date: 2007

Subjects

Subjects / Keywords: Plant Cell Biology
Cellular Signaling
Gravitropism
Gravistimulation
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: During a plant's lifespan, it is bound to encounter and respond to many stimuli, including changes in orientation with respect to the gravity vector. During harsh storms or gusts of wind, a plant may tip over, and it is necessary for it to right itself in order to continue reasonably normal growth. Many monocots achieve this reorientation by differential growth in specialized discs of tissue along the stem known as pulvini. Following stimulation, the cells in the lower half of the pulvini elongate, leading to an upward curvature of the stem. Gravity perception occurs in specialized cells known as statocytes, which contain amyloplasts that sediment upon reorientation. How this sedimentation leads to a change in growth is still largely unknown. Although recent studies have begun tackling the pieces of the signaling pathway, little is understood about the changes in gene expression that lead to differential growth. Preliminary results of Clore et al. (in preparation) suggest that the iron regulatory protein 1 (irp1) gene is upregulated soon after gravistimulation in maize (Zea mays), and that IRP1 may play a part in the early signaling pathway prior to differential growth. We aimed to further test this hypothesis by measuring the levels of irp1 mRNA at various timepoints after gravistimulation in both upper and lower halves of the pulvinus. To do so, we used the sensitive and quantitative technique of real time reverse-transcriptase polymerase chain reaction (real-time RT-PCR). We have determined that there appears to be a pattern of synchronous rises and falls of irp1 transcript levels in both the upper and lower halves of the pulvinus, whereas gene expression is reasonably steady in vertical pulvini and in gravistimulated internode tissue. Somewhat unexpectedly, modest increases in expression did occur in node tissues as well as leaf sheath tissue immediately around the pulvinus. Possible explanations for this result are discussed. We hypothesize that IRP1 may be part of a cascade that leads from gravity perception to asymmetrical growth and propose future experiments to study this possibility further.
Statement of Responsibility: by Sarah M. Doore
Thesis: Thesis (B.A.) -- New College of Florida, 2007
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Clore, Amy

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2007 D69
System ID: NCFE003761:00001

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