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An Investigation of the Potential Role of Brassinosteroids in Carpel Fusion in Madagascar Periwinkle (Catharanthus roseus L.)

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003658/00001

Material Information

Title: An Investigation of the Potential Role of Brassinosteroids in Carpel Fusion in Madagascar Periwinkle (Catharanthus roseus L.)
Physical Description: Book
Language: English
Creator: Kahn, Barbara
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2006
Publication Date: 2006

Subjects

Subjects / Keywords: Brassinosteroids
Cell Signaling
Plant Biology
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: During floral ontogeny in Madagascar periwinkle (Catharanthus roseus L.), the two distinct carpels arising from the meristem fuse to form a single gynoecium. As part of this process, approximately 400 epidermal cells on the contacting (adaxial) surfaces change fate and become parenchyma cells. Previous work has shown that a diffusible factor (or factors) is exchanged between the carpels to initiate these changes, but the chemical nature of the factor(s) is unknown. We have found that treatment of the factor with proteinase K did not change its ability to elicit this fate change. This result led us to consider non-proteinaceous substances, such as lipids. Therefore, we treated cuttings with spironolactone, which has been shown by others to inhibit the action of steroidal plant hormones, known as brassinosteroids. Such treatment resulted in inhibition of fusion, in some samples, or incomplete fusion in other samples. In addition, 24-epibrassinolide applied directly to the carpel abaxial surface appeared to induce features of the cell fate change. Future work in the laboratory will be aimed at further testing the hypothesis that brassinosteroids play an important role in carpel fusion in periwinkle.
Statement of Responsibility: by Barbara Kahn
Thesis: Thesis (B.A.) -- New College of Florida, 2006
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Clore, Amy

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2006 K1
System ID: NCFE003658:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003658/00001

Material Information

Title: An Investigation of the Potential Role of Brassinosteroids in Carpel Fusion in Madagascar Periwinkle (Catharanthus roseus L.)
Physical Description: Book
Language: English
Creator: Kahn, Barbara
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2006
Publication Date: 2006

Subjects

Subjects / Keywords: Brassinosteroids
Cell Signaling
Plant Biology
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: During floral ontogeny in Madagascar periwinkle (Catharanthus roseus L.), the two distinct carpels arising from the meristem fuse to form a single gynoecium. As part of this process, approximately 400 epidermal cells on the contacting (adaxial) surfaces change fate and become parenchyma cells. Previous work has shown that a diffusible factor (or factors) is exchanged between the carpels to initiate these changes, but the chemical nature of the factor(s) is unknown. We have found that treatment of the factor with proteinase K did not change its ability to elicit this fate change. This result led us to consider non-proteinaceous substances, such as lipids. Therefore, we treated cuttings with spironolactone, which has been shown by others to inhibit the action of steroidal plant hormones, known as brassinosteroids. Such treatment resulted in inhibition of fusion, in some samples, or incomplete fusion in other samples. In addition, 24-epibrassinolide applied directly to the carpel abaxial surface appeared to induce features of the cell fate change. Future work in the laboratory will be aimed at further testing the hypothesis that brassinosteroids play an important role in carpel fusion in periwinkle.
Statement of Responsibility: by Barbara Kahn
Thesis: Thesis (B.A.) -- New College of Florida, 2006
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Clore, Amy

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2006 K1
System ID: NCFE003658:00001

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