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A Study of RNA Helicase A Expression in Caenorhabditis elegans Using a rha-l-gfp Reporter

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003565/00001

Material Information

Title: A Study of RNA Helicase A Expression in Caenorhabditis elegans Using a rha-l-gfp Reporter
Physical Description: Book
Language: English
Creator: Samoilova, Eugenia
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2005
Publication Date: 2005

Subjects

Subjects / Keywords: RNA Helicase A
Fluorescence
GFP
C. elegans
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: RNA helicase A (RHA) belongs to the DExH family of helicases and is implicated in the events of nucleic acid metabolism. Many studies are underway to determine the exact functions RHA performs in the cell and the importance of this protein to normal cell proliferation. It is a great advantage that this protein is ubiquitously expressed in a variety of lower organisms as it provides for easier modes of study. However, very little is known about RHA-l functions in C. elegans, an excellent model organism for biological research. To be able to utilize C. elegans in the study of the functions of RHA-1, an accurate reporter system is necessary. The goal of this project was to develop a GFP-containing reporter system. Once the reporter is constructed, it was then injected into the C. elegans germline to allow for the formation of an extrachromosomal array. To test the incorporation of the reporter into C. elegans, various tests were performed on the worms. Successful cloning of this new GFP-containing reporter into C. elegans should allow for easy detection of RHA-1 in vivo due to GFP fluorescence. This ultimately should allow for determination of RHA localization during different stages of the development of C. elegans. Our results indicated that even though a partially functional RHA-1 was present in the transgenic worms, RHA-1 could not be detected by GFP fluorescence. We concluded that further study is necessary to elucidate the reasons behind diminished RHA-1 function and how it can be augmented.
Statement of Responsibility: by Eugenia Samoilova
Thesis: Thesis (B.A.) -- New College of Florida, 2005
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2005 S1
System ID: NCFE003565:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003565/00001

Material Information

Title: A Study of RNA Helicase A Expression in Caenorhabditis elegans Using a rha-l-gfp Reporter
Physical Description: Book
Language: English
Creator: Samoilova, Eugenia
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2005
Publication Date: 2005

Subjects

Subjects / Keywords: RNA Helicase A
Fluorescence
GFP
C. elegans
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: RNA helicase A (RHA) belongs to the DExH family of helicases and is implicated in the events of nucleic acid metabolism. Many studies are underway to determine the exact functions RHA performs in the cell and the importance of this protein to normal cell proliferation. It is a great advantage that this protein is ubiquitously expressed in a variety of lower organisms as it provides for easier modes of study. However, very little is known about RHA-l functions in C. elegans, an excellent model organism for biological research. To be able to utilize C. elegans in the study of the functions of RHA-1, an accurate reporter system is necessary. The goal of this project was to develop a GFP-containing reporter system. Once the reporter is constructed, it was then injected into the C. elegans germline to allow for the formation of an extrachromosomal array. To test the incorporation of the reporter into C. elegans, various tests were performed on the worms. Successful cloning of this new GFP-containing reporter into C. elegans should allow for easy detection of RHA-1 in vivo due to GFP fluorescence. This ultimately should allow for determination of RHA localization during different stages of the development of C. elegans. Our results indicated that even though a partially functional RHA-1 was present in the transgenic worms, RHA-1 could not be detected by GFP fluorescence. We concluded that further study is necessary to elucidate the reasons behind diminished RHA-1 function and how it can be augmented.
Statement of Responsibility: by Eugenia Samoilova
Thesis: Thesis (B.A.) -- New College of Florida, 2005
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2005 S1
System ID: NCFE003565:00001

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