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Incorporation of Active Lactate Dehydrogenase into Polyelectrolyte Multilayer Assemblies

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003537/00001

Material Information

Title: Incorporation of Active Lactate Dehydrogenase into Polyelectrolyte Multilayer Assemblies
Physical Description: Book
Language: English
Creator: Martin, Rebecca
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2005
Publication Date: 2005

Subjects

Subjects / Keywords: Lactate Dehydrogenase
Polyelectrolyte Multilayers
Electrostatic Self-Assembly
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: The goal of this research is to assemble novel polyelectrolyte thin film systems comprised of a polyanion, a polycation and a biologically active enzyme, lactate dehydrogenase (LDH). The function of this catalytic device will be monitored as the conversion of pyruvate to lactate is catalyzed by LDH. This work investigates the immobilization, catalytic activity, and reusability of LDH in polyelectrolyte multilayer film assemblies constructed via electrostatic self-assembly and spin-assembly. Enzymes, and other biomolecules, have been successfully integrated into polyelectrolyte multilayer assemblies in the past but little attention has been given to investigating conditions which prevent leaching of the enzyme out of the film assemblies into aqueous solution. Results presented in this thesis demonstrate that electrostatically bound LDH between polycation layers of poly(ethylenimine) (PEI) remains catalytically active without leaching into solution. This has significant application as a model system for biosensors or surface catalytic devices.
Statement of Responsibility: by Rebecca Martin
Thesis: Thesis (B.A.) -- New College of Florida, 2005
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Johal, Malkait

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2005 M3
System ID: NCFE003537:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003537/00001

Material Information

Title: Incorporation of Active Lactate Dehydrogenase into Polyelectrolyte Multilayer Assemblies
Physical Description: Book
Language: English
Creator: Martin, Rebecca
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2005
Publication Date: 2005

Subjects

Subjects / Keywords: Lactate Dehydrogenase
Polyelectrolyte Multilayers
Electrostatic Self-Assembly
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: The goal of this research is to assemble novel polyelectrolyte thin film systems comprised of a polyanion, a polycation and a biologically active enzyme, lactate dehydrogenase (LDH). The function of this catalytic device will be monitored as the conversion of pyruvate to lactate is catalyzed by LDH. This work investigates the immobilization, catalytic activity, and reusability of LDH in polyelectrolyte multilayer film assemblies constructed via electrostatic self-assembly and spin-assembly. Enzymes, and other biomolecules, have been successfully integrated into polyelectrolyte multilayer assemblies in the past but little attention has been given to investigating conditions which prevent leaching of the enzyme out of the film assemblies into aqueous solution. Results presented in this thesis demonstrate that electrostatically bound LDH between polycation layers of poly(ethylenimine) (PEI) remains catalytically active without leaching into solution. This has significant application as a model system for biosensors or surface catalytic devices.
Statement of Responsibility: by Rebecca Martin
Thesis: Thesis (B.A.) -- New College of Florida, 2005
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Johal, Malkait

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2005 M3
System ID: NCFE003537:00001

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