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Characterization of Epidermal Cell Dedifferentiation during Carpel Fusion in Madagascar Periwinkle

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003365/00001

Material Information

Title: Characterization of Epidermal Cell Dedifferentiation during Carpel Fusion in Madagascar Periwinkle
Physical Description: Book
Language: English
Creator: Durbak, Amanda R.
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2004
Publication Date: 2004

Subjects

Subjects / Keywords: Plant Biology
Dedifferentiation
Development (Plant)
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: In some species, organ fusions occur during normal floral development. In Madagascar periwinkle (Catharanthus roseus), two carpels undergo postgenital fusion, resulting in the dedifferentiation of approximately 400 epidermal cells into parenchyma cells. Dedifferentiation and fusion are triggered by an unknown factor(s), which appears to be soluble and is able to pass through the plasma membranes, walls, and cuticles of the carpel epidermal cells. In order to find better ways of collecting factor for later identification, factor was both trapped in porous polycarbonate barriers placed in between the carpels (as has been previously reported) or in agarsolidified medium into which cultured carpels secreted factor. The trapped factor was applied to the abaxial surface of different sets of carpels, and dedifferentiation was induced in areas where it was not normally seen. In addition, agar blocks that had been in contact only with pre-fusion carpels appeared to induce epidermal cell walls on this abaxial surface to become wavy, perhaps indicating a wall modification effect. Also, RNA was extracted from carpels at different stages of fusion, and double-stranded cDNA was generated using BD SMARTtm PCR. The resulting cDNA populations were then subjected to suppressive-subtractive hybridization PCR, and fragments were amplified that should correspond to genes that are differentially expressed between the pre-fusion and fusing stages. Subsequent purification, sequencing, and comparison of these fragments to known sequences should provide better knowledge as to how the dedifferentiation process works in this system. Future work will also focus on the identification of the molecules secreted by both pre- and fusing stage carpels.
Statement of Responsibility: by Amanda R. Durbak
Thesis: Thesis (B.A.) -- New College of Florida, 2004
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Clore, Amy

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2004 D9
System ID: NCFE003365:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003365/00001

Material Information

Title: Characterization of Epidermal Cell Dedifferentiation during Carpel Fusion in Madagascar Periwinkle
Physical Description: Book
Language: English
Creator: Durbak, Amanda R.
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2004
Publication Date: 2004

Subjects

Subjects / Keywords: Plant Biology
Dedifferentiation
Development (Plant)
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: In some species, organ fusions occur during normal floral development. In Madagascar periwinkle (Catharanthus roseus), two carpels undergo postgenital fusion, resulting in the dedifferentiation of approximately 400 epidermal cells into parenchyma cells. Dedifferentiation and fusion are triggered by an unknown factor(s), which appears to be soluble and is able to pass through the plasma membranes, walls, and cuticles of the carpel epidermal cells. In order to find better ways of collecting factor for later identification, factor was both trapped in porous polycarbonate barriers placed in between the carpels (as has been previously reported) or in agarsolidified medium into which cultured carpels secreted factor. The trapped factor was applied to the abaxial surface of different sets of carpels, and dedifferentiation was induced in areas where it was not normally seen. In addition, agar blocks that had been in contact only with pre-fusion carpels appeared to induce epidermal cell walls on this abaxial surface to become wavy, perhaps indicating a wall modification effect. Also, RNA was extracted from carpels at different stages of fusion, and double-stranded cDNA was generated using BD SMARTtm PCR. The resulting cDNA populations were then subjected to suppressive-subtractive hybridization PCR, and fragments were amplified that should correspond to genes that are differentially expressed between the pre-fusion and fusing stages. Subsequent purification, sequencing, and comparison of these fragments to known sequences should provide better knowledge as to how the dedifferentiation process works in this system. Future work will also focus on the identification of the molecules secreted by both pre- and fusing stage carpels.
Statement of Responsibility: by Amanda R. Durbak
Thesis: Thesis (B.A.) -- New College of Florida, 2004
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Clore, Amy

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2004 D9
System ID: NCFE003365:00001

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