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Attenuated Total Reflectance Infrared Spectroscopy of Synthetic Biological Membranes under UV Peroxidative Stress

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003346/00001

Material Information

Title: Attenuated Total Reflectance Infrared Spectroscopy of Synthetic Biological Membranes under UV Peroxidative Stress
Physical Description: Book
Language: English
Creator: Browne, William
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2004
Publication Date: 2004

Subjects

Subjects / Keywords: Synthetic Biological Membrane
ATR-FTIR
Ozone
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: UV-patteming is a technique that has proved useful in creating stable holes of a desired two dimensional architecture in self-assembled lipid bilayers. However, conclusive chemical evidence that the UV-patterning process destroys the lipids where these holes are created has yet to be provided. The original purpose of this research was to establish that lipids arranged in multilayers are degraded by UV peroxidative stress. This work demonstrates that Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a convenient method to determine whether hydrated lipids tethered to a solid hydrophilic substrate are fully oxidized using the UV-patteming technique. In this work, multilayers of five lipids are constructed at the interface between a ZnSe solid substrate and an aqueous phase. The ZnSe acts both as a hydrophilic substrate and as an internal reflection element for ATR-FTIR studies. Infrared spectra of the multilayer films as a function of UV exposure time were then collected in situ. Direct chemical proof of the degradation of all the lipid multilayers exposed to UV light was provided. This work also established elevated levels of ozone in aqueous solution after exposure to UV light. Ozone is known to play an important role in the oxidative degradation of the lipid multilayers.
Statement of Responsibility: by William Browne
Thesis: Thesis (B.A.) -- New College of Florida, 2004
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Johal, Malkiat

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2004 B88
System ID: NCFE003346:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003346/00001

Material Information

Title: Attenuated Total Reflectance Infrared Spectroscopy of Synthetic Biological Membranes under UV Peroxidative Stress
Physical Description: Book
Language: English
Creator: Browne, William
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2004
Publication Date: 2004

Subjects

Subjects / Keywords: Synthetic Biological Membrane
ATR-FTIR
Ozone
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: UV-patteming is a technique that has proved useful in creating stable holes of a desired two dimensional architecture in self-assembled lipid bilayers. However, conclusive chemical evidence that the UV-patterning process destroys the lipids where these holes are created has yet to be provided. The original purpose of this research was to establish that lipids arranged in multilayers are degraded by UV peroxidative stress. This work demonstrates that Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a convenient method to determine whether hydrated lipids tethered to a solid hydrophilic substrate are fully oxidized using the UV-patteming technique. In this work, multilayers of five lipids are constructed at the interface between a ZnSe solid substrate and an aqueous phase. The ZnSe acts both as a hydrophilic substrate and as an internal reflection element for ATR-FTIR studies. Infrared spectra of the multilayer films as a function of UV exposure time were then collected in situ. Direct chemical proof of the degradation of all the lipid multilayers exposed to UV light was provided. This work also established elevated levels of ozone in aqueous solution after exposure to UV light. Ozone is known to play an important role in the oxidative degradation of the lipid multilayers.
Statement of Responsibility: by William Browne
Thesis: Thesis (B.A.) -- New College of Florida, 2004
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Johal, Malkiat

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2004 B88
System ID: NCFE003346:00001

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