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Site-Directed Mutagenesis of a Single-Nucleotide Mutation in Familial Atrial Fibrillation

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003329/00001

Material Information

Title: Site-Directed Mutagenesis of a Single-Nucleotide Mutation in Familial Atrial Fibrillation
Physical Description: Book
Language: English
Creator: Zhuang, Hanyi
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2003
Publication Date: 2003

Subjects

Subjects / Keywords: Atrial Fibrillation
Overlap Extension Mutagenesis
Polymerase Chain Reaction
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Atrial fibrillation is the most common form of cardiac arrhythmias with a morbidity of 0.3% to 0.4% in the adult population. The majority of the cases of AF are related to cardiovascular abnormalities. Idiopathic atrial fibrillation is defined as the specific kind of atrial fibrillation without organic or structural heart diseases. The hereditary pattern of this familial form of the disease has been characterized as autosomal dominant. Clinical evaluations and genomic analysis of a Chinese kindred with familial idiopathic AF has identified KvLQT1, a gene ericoding a voltage-sated potassium channel in human cardiac myocytes on chromosome 11, as the potential disease gene. This paper describes the site-directed mutagenesis procedures used to produce mutated KvLQTl-containing plasmids for future electrophysiological characterization in Xenopus oocytes. Alterations in the electrophysiological properties of the mutated ion channels can be used to confirm the molecular pathogenesis of atrial fibrillation.
Statement of Responsibility: by Hanyi Zhuang
Thesis: Thesis (B.A.) -- New College of Florida, 2003
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2003 Z63
System ID: NCFE003329:00001

Permanent Link: http://ncf.sobek.ufl.edu/NCFE003329/00001

Material Information

Title: Site-Directed Mutagenesis of a Single-Nucleotide Mutation in Familial Atrial Fibrillation
Physical Description: Book
Language: English
Creator: Zhuang, Hanyi
Publisher: New College of Florida
Place of Publication: Sarasota, Fla.
Creation Date: 2003
Publication Date: 2003

Subjects

Subjects / Keywords: Atrial Fibrillation
Overlap Extension Mutagenesis
Polymerase Chain Reaction
Genre: bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, terriorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract: Atrial fibrillation is the most common form of cardiac arrhythmias with a morbidity of 0.3% to 0.4% in the adult population. The majority of the cases of AF are related to cardiovascular abnormalities. Idiopathic atrial fibrillation is defined as the specific kind of atrial fibrillation without organic or structural heart diseases. The hereditary pattern of this familial form of the disease has been characterized as autosomal dominant. Clinical evaluations and genomic analysis of a Chinese kindred with familial idiopathic AF has identified KvLQT1, a gene ericoding a voltage-sated potassium channel in human cardiac myocytes on chromosome 11, as the potential disease gene. This paper describes the site-directed mutagenesis procedures used to produce mutated KvLQTl-containing plasmids for future electrophysiological characterization in Xenopus oocytes. Alterations in the electrophysiological properties of the mutated ion channels can be used to confirm the molecular pathogenesis of atrial fibrillation.
Statement of Responsibility: by Hanyi Zhuang
Thesis: Thesis (B.A.) -- New College of Florida, 2003
Electronic Access: RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography: Includes bibliographical references.
Source of Description: This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Local: Faculty Sponsor: Walstrom, Katherine

Record Information

Source Institution: New College of Florida
Holding Location: New College of Florida
Rights Management: Applicable rights reserved.
Classification: local - S.T. 2003 Z63
System ID: NCFE003329:00001

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