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CIRCADIAN REGULATION OF OXIDATIVE FAT METABOLISM IN C2C12 MYOTUBES

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Material Information

Title:
CIRCADIAN REGULATION OF OXIDATIVE FAT METABOLISM IN C2C12 MYOTUBES
Physical Description:
Book
Language:
English
Creator:
Bacsik, David
Publisher:
New College of Florida
Place of Publication:
Sarasota, Fla.
Publication Date:

Thesis/Dissertation Information

Degree:
Bachelor's ( B.A.)
Degree Grantor:
New College of Florida
Degree Divisions:
Natural Sciences
Area of Concentration:
Biology
Faculty Sponsor:
Clore, Amy

Subjects

Genre:
bibliography   ( marcgt )
theses   ( marcgt )
government publication (state, provincial, territorial, dependent)   ( marcgt )
born-digital   ( sobekcm )
Electronic Thesis or Dissertation

Notes

Abstract:
The molecular circadian clock consists of a transcription-translation feedback loop that drives the rhythmic expression of hundreds of genes. The rate-limiting enzyme of the NAD+ salvage pathway, nicotinamide phosphoribosyltransferase (NAMPT), is a known target of the molecular circadian clock. Recently, the Bass Lab group at Northwestern University elucidated an NAD+ dependent pathway connecting the molecular circadian clock to fatty acid oxidation in mouse liver. Circadian regulation of oxidative fat metabolism coordinates metabolic activities with circadian feeding and fasting behaviors. To facilitate further study of this pathway, a mouse skeletal muscle cell line, C2C12, was tested as a model system for studying fat metabolism and circadian clock function. Rhythms of NAD+ concentration and mitochondrial respiration were observed in synchronized C2C12 myotubes. NAMPT was found to have substantial influence on cellular NAD+ concentrations, however, NAMPT expression as measured by qPCR varied little throughout the day. These data suggest that, although further investigation into the regulation of NAMPT activity is required, the C2C12 cell line may be a useful model for the study of circadian metabolism.
Statement of Responsibility:
by David Bacsik
Thesis:
Thesis (B.A.) -- New College of Florida, 2014
General Note:
Online version not currently available.
General Note:
RESTRICTED TO NCF STUDENTS, STAFF, FACULTY, AND ON-CAMPUS USE
Bibliography:
Includes bibliographical references.
General Note:
This bibliographic record is available under the Creative Commons CC0 public domain dedication. The New College of Florida Libraries, as creator of this bibliographic record, has waived all rights to it worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
General Note:
Faculty Sponsor: Clore, Amy

Record Information

Source Institution:
New College of Florida
Holding Location:
New College of Florida
Rights Management:
Applicable rights reserved.
Classification:
S.T. 2014 B33
System ID:
AA00024708:00001

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